產(chǎn)品詳情
簡單介紹：
重組人血小板源生長Factor-BB蛋白與其它公司提供的重組蛋白不同，rHuPDGF-BB蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人血小板源生長Factor-BB蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人血小板源生長Factor-BB蛋白
| Synonyms | Glioma-derived Growth Factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-2, Platelet-derived Growth Factor Beta Polypeptide, Becaplermin |
| Species | Human |
| Accession | P01127 |
| GeneID | 5155 |
| Source | 重組人血小板源生長Factor-BB蛋白Escherichia coli. |
| Molecular Weight | Approximately 24.8 kDa, a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain). |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg. |
| Physical Appearance | 重組人血小板源生長Factor-BB蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人血小板源生長Factor-BB蛋白 | |
| SDS-PAGE |
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| Reference |
1. Hart CE, Bailey M, Curtis DA, et al. 1990. Biochemistry, 29: 166-72. 2. Heidaran MA, Yu JC, Jensen RA, et al. 1992. J Biol Chem, 267: 2884-7. 3. Haber M, Cao Z, Panjwani N, et al. 2003. Vet Ophthalmol, 6: 211-7. 4. Matei D, Kelich S, Cao L, et al. 2007. Cancer Biol Ther, 6: 1951-9. 5. Chung R, Foster BK, Zannettino AC, et al. 2009. Bone, 44: 878-85. 6. Sufen G, Xianghong Y, Yongxia C, et al. 2011. Cell Biol Int, 35: 545-51. |
| Background | 重組人血小板源生長Factor-BB蛋白Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |
