產(chǎn)品詳情
簡單介紹：
重組人粒細胞集落刺激因子蛋白與其它公司提供的重組蛋白不同，rHuG-CSF蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人粒細胞集落刺激因子蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人粒細胞集落刺激因子蛋白
| Synonyms | CSF-3, MGI-1G, GM-CSF beta, Pluripoietin |
| Species | Human |
| Accession | P09919 |
| GeneID | 1440 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人粒細胞集落刺激因子蛋白Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人粒細胞集落刺激因子蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0. |
| Endotoxin | Less than 1 EU/μg of rHuG-CSF as determined by LAL method. |
| Reconstitution | 重組人粒細胞集落刺激因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人粒細胞集落刺激因子蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30. 2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33. 3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8. 4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338. 5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9. |
| Background | Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition it may function in some adhesion or recognition events at the cell surface. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (a.a.) precursor proteins, have been isolated. Both proteins have a 30 a.a. signal peptide and have identical amino acid sequences except for a three a.a. insertion (deletion) at the 35th a.a. residue from the N-terminus of the mature protein. Human G-CSF is 73 % identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. |
